Catalog Number:82023

IntelliPlex™ ALK Rearrangement Kit

The IntelliPlex ALK Rearrangement Kit is a qualitative molecular assay for the aberrant gene fusions detection on EML4-ALK, KIF5B-ALK, and TFG-ALK transcripts. The kit utilizes one-step RT-PCR and PlexBio's core πCode technology which enables 24 ALK variants to be identified in a single well using RNA samples extracted from formalin-fixed paraffin-embedded tissues (FFPET).

FEATURES
  • Detect 24 ALK fusion variants in one well test
  • Highly sensitive (5~1209 copies) to detect low levels of RNA variants
  • Only ≥50ng RNA sample required
  • Time to results in <6 hours for 96 samples per run (sample preparation included)
multiplexing

Multi-targets Detection

Detectable 24 ALK fusion variants in one well
sample

Low Sample Input

Only ≥50ng RNA sample extracted from FFPE tissues required
high sensitivity

High Sensitivity

Detects low levels of RNA variants with a high sensitivity
quick turnaround time

Quick Turnaround Time

Sample to results in <6 hours with automated reporting 
Overview
The IntelliPlex ALK Rearrangement Kit is an in-vitro molecular test based on πCode technology and PlexBio's instrument platform to identify 24 fusion variants in ALK gene. πCode technology enables the multi-variants detection in single-well with RNA samples derived from formalin-fixed paraffin-embedded (FFPE) human tissues. The kit provides all necessary optimized reagents for the sensitive detection of low levels of RNA variants in a background of wild-type RNA by one-step RT-PCR and simultaneous PCR amplification of target cDNA. The testing results could deliver crucial molecular status to clinical evaluation.

Principle of πCode Multiplexing

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