πCode Technology

Performing high multiplex assays doesn't need to be complex. PlexBio offers a series of convenient, pre-activated πCode™ MicroDisc products as a simple solution for the conjugation of virtually any capture reagent. Simply incubate your ligand in the appropriate buffer controlled pH and temperature conditions.  Pre-activated πCode™ MicroDiscs are ready to use for your own tests, and can be applied in both molecular and immunoassays.

Each πCode MicroDisc, with it's distinct circular image pattern, corresponds to the specific capture agent conjugated to the disc surface. This capture agent allows the capture and detection of targets from a sample.  Virtually any probe used in clinical diagnostics can be conjugated to πCode MicroDiscs, including DNA, RNA. antigens, antibodies, proteins, or chemical compounds.

Why Multiplexing?

Multiplexing provides an optimized solution for running multi-assay tests at an affordable cost.  Unlike traditional methods, a multiplex assay can provide results for multiple targets in a single reaction, saving time, labor, reagents, tissue samples, and thereby overall reducing laboratory costs.

How it Works

In order to perform a biological test, πCode MicroDiscs with desired capture probes were pooled together in a single well and reacted with patient sample.  For example, there are 40 mutations/targets to be detected in our IntelliPlex™ EGFR Mutation Kit, hence, the number of different patterns of πCode MicroDiscs present in the assay is 40 types.  The assay then is followed by hybridization, fluorescent labeling, and detected by optical imaging fluorescence analyzer PlexBio™ 100 - which uses the CCD camera to read the distinct image patterns under bright field and quantifies the target under dark field.  PlexBio™ 100 Fluorescent Analyzer measures the fluorescence signal intensity (MFI) releases by each target and provides a comprehensive report in a user-friendly interface.

Where can it be applied?    


Order Information

CATEGORYPRODUCT NAMEPACKAGE SIZECAT. No. 
ΠCODE™ΠCode™ MicroDisc, Pre-activated
 10k Discs
 80131
ΠCODE™ MICRODISC, PRE-ACTIVATED 20K DISCS 80132
ΠCODE™ MICRODISC, PRE-ACTIVATED(10-Plex Sampler Kit / 20k Discs Each)
 80133
ΠCODE™ MICRODISC, PRE-ACTIVATED  (10-PLEX SAMPLER KIT / 10K DISCS EACH) 80134
 Buffers ΠCode™ Coupling Buffer Set
 1 Unit 80214
 ACCESSORIES PLEXBIO™  MAGNeTIC STAND
  1 UNIT 80015

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