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Development of an Ultrasensitive HIV-1 DNA Detection Assay Based on an Automated πCode End-Point PCR System

Kazuo Suzuki, Jui-Yu Hu, et al.  JAIDS HIV Treat. 2019 volume 1, issue 2, p46~65

We have developed a novel end-point PCR assay based on precision image pi-code (πCode) MicroDiscs detection platform. The newly developed “πCode assay” showed 100% sensitivity, while the real-time PCR assay showed 92.3%, in a head-to-head comparison using blood samples from 39 HIV-1 infected clinic patients, with a stable fully suppressed plasma viral-load (<20 copies/mL) for more than 2 years..... 

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Comprehensive Coverage of Lung Cancer Somatic Mutations for Liquid Biopsy Testing by πCode™ Technology

Shengfong Huang 1, Shian-Pin Hu 1 , Aidan Coe 1   , H-Eugene Liu 2 , Yen-Lin Chen  3 , Stuart M. Palmer  1 , Chin-shiou Huang  1 , Dean Tsao 1   

PlexBio Co., Ltd., Taipei, Taiwan; Department of Hematology and Oncology, Municipal Wanfang Hospital, Taipei, Taiwan; Department of Pathology, Fu-Jen Catholic University, New Taipei, Taiwan 

IntelliPlex™ EGFR Mutation Kit, is designed to assess the status of 40 somatic mutations of the EGFR gene in tissue or liquid biopsy samples. In combination with SelectAmp, a mutation-enriching PCR amplification that dramatically increases mutation detection sensitivities, achievement of sensitivities up to 0.1% is common in many mutation points making this analytic approach ideally suited for liquid biopsy assays.

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A Novel High Plex Analysis System with Molecular and Immunoassay Capabilities

Shian-Pin Hu 1, Jui-Yu Hu 1, Stuart M. Palmer 1, Chin-shiou Huang 1, Dean Tsao 1

PlexBio Co., Ltd., Taipei, Taiwan

PlexBio’s πCode™ MicroDisc technology is currently the largest multiplexing platform commercially available, capable of generating over 16,000 circular image patterns. By using this higher order multiplexing platform, PlexBio is able to offer highly sensitive and specific results for both immuno and molecular diagnostic formats from very low input sample volumes – saving laboratories time and labor.

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Multiplex Assays for Cancer Management Using πCode MicroDisc Technology

Shian-Pin Hu 1, Yi-Ling Hsieh 1, Yao-Yun Cheng 1, Aidan Coe 1, Stuart M. Palmer 1, Chin-Shiou Huang 1, Dean Tsao 1

1 PlexBioCo., Ltd., Taipei, Taiwan

PlexBio’s IntelliPlex™ Lung Cancer Panel, has been shown the ability to assess 55 gene mutations and gene rearrangements (36 DNA single gene mutation sets and 19 RNA gene rearrangement sets) from either from FFPE or clinical liquid biopsy specimens associated with NSCLC. This two-reaction panel was developed using the existing reagent sets of PlexBio’s stand-alone CDx products for EGFR, KRAS, BRAF, NRAS, PIK3CA, ALK and ROS1 gene mutations and gene rearrangement assays.

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IntelliPlex™ EGFR Mutation Kit

IntelliPlex™ BRAF Mutation Kit

IntelliPlex™ PIK3CA Mutation Kit

IntelliPlex™ NRAS Mutation Kit

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IntelliPlex™ ALK Rearrangement Kit

IntelliPlex™ ROS1 Rearrangement Kit

IntelliPlex™ RET/NTRK1 Rearrangement Kit

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IntelliPlex™ EGFR Mutation Kit

IntelliPlex™ BRAF Vˊ600 Mutation Kit

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IntelliPlex™ ALK Rearrangement Kit

IntelliPlex™ ROS1 Rearrangement Kit

IntelliPlex™ RET/NTRK1 Rearrangement Kit

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PlexBio™100 Fluorescent Analyzer

IntelliPlex™ 1000 πCode Processor

IntelliPlex™ ALK Rearrangement Kit

IntelliPlex™ EGFR Mutation Kit

IntelliPlex™ BRAF V600 Mutation Kit

IntelliPlex™ ROS1 Rearrangement Kit

IntelliPlex™ RET/NTRK1 Rearrangement Kit

IntelliPlex™ NRAS Mutation Kit

IntelliPlex™ KRAS Mutaion Plus Kit

IntelliPlex™ PIK3CA Mutation Kit

IntelliPlex™ Lung Cancer Panel

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